fibrillosum, A.soleirolioides and you may A beneficial.polyphyllum, as a result of the changes noticed in the 2C DNA contents of these types of species, compliment of their storage for 2 ages inside silica (see above). Ploidy accounts and 1Cx-thinking was basically inferred for everybody other subclades analysed (Dining table 4 and you may Figure step three).
Such as for example, weighed against the tiny 1Cx-viewpoints on A
A summarized phylogenetic tree of one’s Asplenium monanthes cutting-edge and you can associated lineages [because demonstrated in the 31] utilizing the inferred ploidy membership (considering wrote chromosome studies), imply holoploid genome dimensions (1C-value; gray taverns), and inferred monoploid genome proportions (1C x -value; black colored pubs) as well as the indicate spore length (?m) of each and every working unit. Posterior part assistance >0.8 is revealed. To possess diploids just black taverns exist since 1C-worth is equal to the latest 1Cx-really worth. Fundamental error bars are included to own 1C-value and you will 1Cx-worth mode, apart from lineages represented from the one specimen only. Forest is grounded predicated on Dyer ainsi que al. . Specifics of the container plots to have spore proportions are given inside the latest legend to find step 1.
From inside the An effective.resiliens clade, some other ploidy account had been inferred towards the several An effective.aff.heterochroum specimens. You to definitely specimen out of An effective.aff.heterochroum (RD75; 2C = pg) got an identical 2C-well worth so you’re able to A.resiliens (mean 2C = pg), a species said to be triploid, recommending one to RD75 could also be an effective triploid. In contrast, another A.aff.heterochroum specimen analysed (RD9a) got a significantly highest 2C-well worth ( pg) and an effective 1Cx-worthy of everything four times one inferred getting RD75, hence lead to the inference this specimen is actually good tetraploid.
The brand new suggestion that all A.monanthes specimens was triploid is based on the newest observation you to definitely, apart from you to definitely declaration of a tetraploid amount, the matters for it varieties was basically 2n = 3x = 108. Under this expectation, the fresh new indicate 1Cx-viewpoints getting MO1 sandwich-clades I, II and you can III is pg, 9.33 grams, and you can 8.95 pg, respectively (Dining table cuatro). Good triploid level is additionally presumed to possess MO2 (I), in line with the 2C-viewpoints, resulting in a mean 1Cx-worth of 9.02 pg. The brand new An excellent.monanthes MO2 (II) sub-clade, which constructed a single sample (RD96), had a considerably straight down 2C-property value pg, therefore we infer this particular specimen may be a good diploid cytotype. This should produce a good 1Cx property value 9.30 pg, which is inside the broad agreement to your monoploid genome systems computed into the kept An effective.monanthes sub-clades (Table 4).
In the An effective.hallbergii, brand new similarity of its mean 2C-worthy of ( pg) and you can mean spore dimensions ( ?m) to that of your triploid Good.monanthes lineages (MO1 and you may MO2.I) indicates so it species is also an excellent triploid. As for the several the newest species, specification.the fall of.1 and you may spec.late.2, it differ in 2C-thinking ( pg and pg respectively) but have likewise small spore designs. He could be already inferred are diploid regardless of if subsequent research are wanted to show otherwise reject it.
However, it’s noted your imply spore measurements of RD96 ( ?m) disputes using this in search of, recommending that specimen is actually a good polyploid
Overall, the results reveal that monoploid genome proportions may differ 2.4-flex (4.66- pg), with little to no type for the 1Cx-values inside clades, however, significant adaptation ranging from many (Table 4 and you may Profile 3). resiliens clade (cuatro.66-4.79 pg), the brand new imply 1Cx-value for diploid A great.formosum (six.81 pg) try markedly high. A much bigger monoploid genome dimensions has also been noted regarding An effective.monanthes clade weighed against the latest Good.resiliens clade. In reality, the biggest monoploid genome versions was found for the https://datingranking.net/tr/antichat-inceleme/ A beneficial.monanthes clade (8.70- pg), with specification.the fall of.2 having the highest 1Cx-really worth ( pg) of all of the, that is over double the suggest monoploid genome sized the fresh An excellent.resiliens clade.